Part:BBa_K2207997:Design

2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 672
  Illegal XhoI site found at 1538
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 85
  Illegal NgoMIV site found at 372
  Illegal AgeI site found at 1847
  Illegal AgeI site found at 1875
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3725
  

Design Notes

We used T7 promoter to improve the enzymes level in cell to synthesis DAPG heterologously.

Source

The coding sequence was pcr from Pseudomonas fluorescens 2P24, promoter and terminator were got from igem stock.